Psilocybe Science with Doma Nunzio
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We step into a mad scientist's laboratory to dive deep into the world of magic mushrooms. Doma Nunzio is the founder of MagicMyco, MycoCoil, The Cultivar Cup and has won the Psilocybin Cup. He is a master of magic mushroom genetics making the best cultivars in the world. We dive into the cutting edge research of the field.
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TRANSCRIPT
Unknown Speaker 0:00 Hey Unknown Speaker 0:11 what's going on, you're listening to the mushroom revival podcast. This is your host Alex store. And he loved going deep into the wonderful, wacky world of mushrooms and fungi. And today we have a beautiful guest DOMA. So what's going on DOMA? Hey Alex, how you doing today? Good. Why don't you give it a little intro to our audience on who you are and what you're up to? Yeah, good day. My name is Dolman Nunzio. I'm the founder of magic myco myco coil and also the cultivar cup. Magic myco is an online based mycology group through Patreon, where we get together on the discord and we chat about projects. We all do the same projects every month, and the members can get significant discounts and benefits and they could also send back their samples to get tested. So we're a community based around research and testing for entheogens mainly for mycology and magic mushrooms. Unknown Speaker 1:12 And I'm seeing your background right now. And probably people can hear it. All the beeps and twirls going on in the background. I have Where are you right now? Yeah, I'm in the magic myco lab. It's I'm in New York City. I'm in my lab. And the machines are running right now. I gotta I'm sorry about that. There's a run, go. Is it really loud? Because like, it's hard to turn it on. It's just got a couple of minutes left, and I'm gonna stop it. So that'll stop in just a minute. We'll Adel. They'll see running in the background. Those are the machines. They're liquid chromatography machines that analyze the samples. Yeah, we'll it'll give context to what we're about to talk about in, in the episode. So you, you've won the psilocybin cup in the past, and you've had multiple strains rank in the top 25. King, you know, what, which cultivars have have won in the past? And what do you think is your success and making these these great cultivars. So the big strain that we're talking about was a tidal wave, there were a couple of other strains submitted to the first psilocybin Cup, which was curated by Oakland hyphae, on 2021. And all three strains did well, but the tidal wave came out on top. And what was the secret to all those winnings is having a great team having a great family, because they helped me with everything, literally, from spores to testing. So like, I send out the spores, we all grow this stuff in different ways that we do. We discuss it, we talk about it, and they send some of them back. So having a good team to help me really has been my secret. Unknown Speaker 2:47 Yeah. And were you surprised that it came out on top? Or were you going in expecting those results? Well, kind of at the time, everyone was telling me that this was like the strongest isolation, like in the world, so I kind of felt like there was a lot of pressure to do good in the competition. But it was just like the icing on the cake when we did because it kind of like validated the past four years worth of research that I was doing. And you know, the way I came up with, that was a little tough. So so it was it was sweet. It was just really sweet. Unknown Speaker 3:25 Awesome, and what what compounds were tested for tidal wave. Unknown Speaker 3:31 So at that first cup, I believe we only tested they tested for psilocybin and psilocin, which are the two main major compounds found in the magic mushrooms, and they're both related. So So psilocybin is the phosphorylated version of slow sand. So once you consume it into your body, it degrades into celestun which is the act of compound that crosses the blood brain barrier. So those are the two major compounds that were looked at but now it's been a couple years since and both my lab and their lab we're looking at a whole bunch of different things you know, NorthBay asst originates and Unknown Speaker 4:13 a normal lowson serotonin Unknown Speaker 4:17 and related derivatives. So we're looking at a lot more now and different things now we're also testing cannabis, other mushrooms like core to SAPs and stuff like that extract. So, you know, I was looking through the, the Unknown Speaker 4:36 multiple psilocybin cups and I noticed that it didn't really matter about the cultivar or, you know, there's there's a couple of cups that I was going through where I saw the same cultivar or have way different results, you know, there's one penis envy cultivar or that it was the same exact cultivar or from the same grower Unknown Speaker 5:00 And one was about three times more potent. So I'm just wondering, you know, besides working on the, the cultivars and making kind of this super strain, so to speak, what other processes are you working on to, you know, from substrate to temperature changes, etc, to make, you know, such a such a heavy hitting hitting mushroom. Exactly. So that's good, good points there. Um, everybody thinks that like, it's all about the genetics, right? So the substrate that you're using the cultivation techniques, how you're cultivating and the substrate that you're using is going to play major roles, and how it comes out. So like you said, with the same strain we've tested, there could be lots of variability. Now, like with a multi sport culture, you might get a lot of variability in inside your tote, meaning that the same fruits from the same harvest can have great variability in their potency. But when you're working with isolated strains, sometimes like with the tidal wave isolations, or the Trinity isolations, they generally tend to be more like the same or more homogeneous, the same throughout the Choat. But you just mentioned that the tidal wave, like even a tidal wave to in certain competitions did very differently. Now in the first one, we would just go in for like, all out, Max, but in the second one, I actually didn't neglect tech, because we wanted to see what the difference at the time there wasn't, we didn't have the testing available ourselves. It wasn't many labs doing it. So we were kind of experimenting in the competition. And we were trying to see what the difference would be and then grow techniques. Just that was a major question at the time. Is it all genetics, or is there other factors too, and with that, one, we put it in an AI grobag with no nutrients whatsoever, it just on a core thing and just left that didn't treat it and missed, it didn't fan it, just like left it and let it do its thing. And it came out considerably lower. Um, having said that, recent trends, testing the isolations, I've noticed more of the sameness within isolated strains. And we did a couple of recent experiments with tidal wave three, and we're gonna have to do a lot more than just like one or even a handful of expect, we're gonna have to do lots of them, look at lots of different totes, lots of different genetics, and over time, we're going to be able to, you know, compile and humanely log data. Unknown Speaker 7:23 And what is the call to bar cup? Also, the cult of our cup, that's my own version of the hyphae cup. So So basically, you know, at that time when, when the hyphae Cup came out, I was looking into doing very much similar things because I don't have a Unknown Speaker 7:41 college degree in like chemistry, or renal biochemistry or anything like that. I'm basically a community scientist and I came from the cannabis industry. I was doing growing cannabis and doing cannabis extraction. So when I came up with some mycology, one of the first questions I asked was, how do we extract and how do we make hybrids? And I got a lot of like, some Unknown Speaker 8:05 back and forth on the on the internet, some good, some not so good advice. You know, I got booted off a lot of service for asking some of these questions. You know, they were telling me like making hybrids, it was impossible because technically, when you're making a multi sport culture, you're making dozens or hundreds of new strains because the the way that the spores work in the primary mycelium and the lifecycle we can talk about that too, a little bit, if you wanted the primary mycelium comes out of the spore. And then it has to mate with another primary mycelium to make a dichorionic or were to mycelium, which then that will be able to fruit. So every time one of the two of those strains comes together, technically, a new strain has been created. So you know, but we still wanted to do what we wanted to try. So I made my own groups. That's how the Patreon got started. And at the time of when the first cup came around, we didn't have any of these good analytical processes available to us. But what it was a goal for us, we wanted to better document our work to show better analytical testing data and stuff like that. So that's one of the reasons why it was so appealing to us to enter that contest. And we were kind of like experimenting with our samples going into it. But since then, we have gotten to I have two of our own HPLC machines here. And we have developed our own methods and made our own cup for originally it was for our members as we were kind of up and coming building this lab two years ago. This is our second year in this new lab, and we've come a long way. And now we've opened up the competition to everyone so members and non members can both get in. You know, if you're a member, you're gonna get the biggest discounts and benefits so so basically, you're either going to pay full price as a non member, you could pay half price as a member, or ID we have two of our top tiers, which includes the testing, those members can actually get into the cultural break. So we have a we call it our Solstice events and we because we do it on the wind Unknown Speaker 10:00 During the summer solstice, the deadline for entry is June 1. And that gives me about three weeks to do all the reports. And on June 21, the reports will be released on my website magic myco and.org, where everybody can check it out. So a little Yeah, we got some cool sponsors here, there's going to be some great prizes, substrates, genetics and trophies, it's going to be really cool. Unknown Speaker 10:24 And has there been any crazy insights from when you started this testing Unknown Speaker 10:31 on? Well, Unknown Speaker 10:33 we're finding more compounds, and we're able to look at basically the whole sample from the beginning to the end, like in other words, basically run it through our column and be able to look at every single thing that comes out of it. That was something I couldn't do in the beginning. And in the beginning, like I mentioned, we were just looking at sloths, and it's psilocybin. As a matter of fact, some of our other compounds were co eluding and those peaks, which means it was kind of grouped up and bunched up. So since then, we've really, you've really dialed it in. And one of the biggest variabilities, between the labs with all of these tests, is not in the equipment is not even in the methods, it's in our sample prep procedures. Most of the time, with chromatography Sample Prep procedures are very simple. And we use 100% methanol, and as long as we do them all the same, you know, everything's going to go line up. But with mushrooms, you know, we're dealing with that cell wall. And people who know mycology and who worked with mushrooms, no, we do like double extractions like double decoctions, where we use water in one setting. And then like an organic like like alcohol, some kind of alcohol, and another wash, because it takes a little bit more effort to kind of bust that cell wall and get all the medicine out of it. Right. So it's not any different in the lab when we're doing Sample Prep procedures. And in the beginning, it was taken me a couple of days to really extract all of the alkaloids out of the sample, which made you know processing times ridiculous, but now we've gotten it down to two hours with some new techniques. Ma ma e with a UAE, it's a microwave assisted extraction, also with ultrasonic assisted extraction, and we can get it done in two hours. So that really, really improves the efficiency and the high throughput. So we can do hundreds of samples, I could do 100 samples at a time and within a few hours, they'll be done. And you know, so as far as efficiency, because because one of the big turnips for some of this testing for the community has been the price is the price points are very expensive, because the consumables the time, like I'm telling you. So having developed these methods and improving them in the way we are, it really improves the efficiency so we can bring the start bringing the price points down, and, and really be able to offer it to everyone in any one and make it affordable. Because I think it's really important, you know, there's some really good home kits on the market now. And we're even trying to develop some reagent kits that you can use for spot testing different drugs, and also for potency analysis. Iraqi Lux is a great one I sell, I'm gonna put the rice distributor of that one on, they give you a good ballpark idea of the amount of tryptamines that you have. But if you really want to know the individual components separate the psilocybin and its losen look at the minus step, you really got to go for the chromatography, testing. And like I said, the prices are coming down. So we're gonna make it more available. You know, we think it's important for people to be batch testing, like you said, there could be lots of variability. But that's why we have this open source approach to our data. So in other words, we share all our methods, we share all our data and put it out there for free. Because we think it's important for others to cultivators, other labs, you know, whether you're a beginner or advanced to get into these topics, because as it becomes legalized, you know, Unknown Speaker 13:58 these things are going to start being implemented into people's practice and they should, and they should so and and for them. Why because so we know how to dose that's the biggest reason so we know how to dose because like you said, you give somebody one gram of magic mushrooms, you don't know if that stream has one milligram of active alkaloids, or if as 40 milligram Well, 50 milligrams of alkaloids, some of the pan science are coming in over 5%, that would be 50 milligrams per gram. So how can you tell somebody hey, I'm gonna take one gram, or we're gonna take this micro dose, when you don't really know how much alcohol so that's why testing is so important. That's why all our processes are so important and the developments and Unknown Speaker 14:39 yeah, absolutely. Unknown Speaker 14:41 And you were talking about that? I think it's pronounced the Unknown Speaker 14:47 Mirror Mirror licks or something like that the harassing miraculous Yeah, yeah, it's French. Or it's German. Okay. Yeah, so I never I always Unknown Speaker 15:00 Write it. And I never actually said it out loud. So Unknown Speaker 15:04 you said, would that test is it total tryptamines like, Are you just getting one number, you basically get to reagents, you get one as a solvent to do your extraction, you follow the instructions, you do your extraction, a little bit of hot water, you mix it with the second reagent, and it'll come back with a color so then you line it up with this color evaluation chart. And this will tell you how strong it is. So basically, it starts from the bottom all the way up to the top and the different gradations of the Brown will tell you how strong it is. Unknown Speaker 15:34 And but now this just gives you total like we said total trip so that's the psilocybin aniseh loosen everything together so if you want to have them so that's good that's good for with your at home cultivators this like 2025 bucks you can do one of these tests know what your batches like but now we got an HPLC testing to download down to like around 100 bucks, it's you know, it's it's competitive it's right there so you know, I advise using these for at home and then when you want to get serious and send the new samples you send them the good ones ah field Unknown Speaker 16:07 do you think that it would ever be possible to test for you know, the the single compounds would you know, something like that test strip Unknown Speaker 16:19 functionality or is this just that's just not possible? Unknown Speaker 16:23 Yeah, we'll make more developments with it every day and different techniques to use them so I do think there'll be better kits on the market are actually cool. Awesome. And you know, Unknown Speaker 16:36 some of the compounds are really not studied at all like they have Syston nor biosystem but a really interesting one a Rogan asin which you're actually featured in a documentary recently how to open great documented it amazing and I brought him on and he he actually introduced us and you know I just wanted to get your perspective on Reagan asin because it's the compound of interest in the wood lovers paralysis which okay you know, you can go into but yeah, what is your thought on this compound and what is doing? Unknown Speaker 17:15 So originates in a Rogen asin, tomatoes, tomatoes, a really nice and and it's derivative, which is or htm t, which we could also look work. Yes, very interesting compounds. I haven't seen much of it in my chromatography. Unknown Speaker 17:32 But I will say what I will say about the wood lovers, Croesus is in looking at that as your essence and the silho Siamese Sire, which is also known as wavy caps, and a couple others. Unknown Speaker 17:48 There's this unknown peak that I don't I still haven't found what it is that comes right after solo sin. Now it's not the four h TMT it's not the third column derivatives. Unknown Speaker 17:59 It's not that I don't know what it is. So there is this unknown. And it's only with these strains with these two particular and maybe one or two other exotic strains, that that pretty consistently. And it's also hard to get a good sample, you know, because they only they only did harvested like once a year in that in that area, in like November, December. So it's like, I gotta line up with somebody who can get me a sample right around then. So this past year, we did happen to get two samples, and I believe one of them had this unknown peak that I still don't know what it is. So could that be the culprit? Probably. Unknown Speaker 18:37 But I still don't know what it is. So more work continues. Unknown Speaker 18:43 And what other compounds do you feel like need a ton more research? Yeah, so So having touched on the miners, so when it comes to any of the miners like origination or biosystem, I also think is extremely important because it's now known to be part of the pathway to psilocybin, right. But these, although these minor compounds have been known to be extremely potent on their own, they can't cross the blood brain barrier on their own. So in order to have that entourage effect or for them to work at all, they would have to be in the presence of the two major alkaloids, the psilocybin solution or an MAO AI in order to become activated. So you know, if you have a sample that has a lot of psilocybin in slawson in it, then sure your miners are gonna play a part. We don't really know what they do. You know, you could go online and look at some preliminary research to effects of what they do. I don't know if I really want to touch on that yet. It's a little too new. But what I will say is that they are not active unless they are in the presence of that those alkaloids with Mao y. Now if they are in the presence of of those compounds, they can win an entourage effect similar to cannabis, you know, with the THC and the CBD and all these other minor compound Unknown Speaker 20:00 As the entourage word phrase has been thrown a lot around a lot entourage effect. And, and, and yes, I have, I'm a participant of some of these substances on my own, you know, in my own my own work and, and I can say that is true like for instance Trinity, which which always seems to have higher biosystem, it just gives me this glow that I don't get like with another strain that maybe didn't have that. So I do think that they that they work, I do think that they are lending effect, but much more research needs to go into and are and the way we design our ports, our reports are in the future. Also, we're taking some things from the cannabis industry. And also I'm getting trying to get certified as a certified ISO 1702 Five laboratory. So in doing that, it's making me come across all these really good processes like, you know, on your computer system, with my inventory and stuff. And also, like some of these other tests and stuff on here, there will be testing a lot of different things. Unknown Speaker 21:07 That's awesome. Training, Phillip, the second. Unknown Speaker 21:12 We were talking about the militaris. Unknown Speaker 21:14 Yeah. And speaking of cannabis, one thing that I stumbled upon with, you know, working with cordyceps militaris was, there's a lot of terpenes going on. And that's not something that is really talked about with mushrooms, but it's talked about all the time with Unknown Speaker 21:32 with cubensis. And we were even coming across it with a bunch of different species functional mushroom species that we were working with currently. But Unknown Speaker 21:43 I'm just curious if anyone has done any terpene testing with cubensis, or is there's any notable ones in there. No, haven't found any terpenes monitor prescribed terpenes yet, there are some special tests. So in other words, it's a different method with different consumables, for beta glucan testing. And for some of the terpene testing, some of the terpene testing might also eat a different machine or a different method was something like that. But one thing that I am finding in doing that long method I was talking about where I could see all the compounds come out in a reverse chromatography in a reverse phase chromatography setting is I found the these unknown peaks, like I told you, I also found Mao wise in very small amounts in a couple of strains. But one thing that I find in every single strain towards the end of the run, and this is a good indicator, how I know that my column is liens is ergosterol. And ergosterol, I think is very important to our research that because there's a direct relation with vitamin D, which is in almost every mushroom, and also the kite and so there's a relation between chitin and ergosterol. And it could also be a relation to potency in the end because you know, cubensis is a very dense and fibers kind of mature, whereas science is is very, very wispy and thin and there's like almost no fibers there. And perhaps that's the reason why that they're they're more potent is because as the fibers and the tissues dry up, it leaves behind those alkaloids and there's just less tissue and less fibers in a pan saya and then there is in a cubensis. So that could possibly be a reason why they're more potent. Yeah, I think the ergosterol is important. And vitamin D also is very important, and it comes out on every single chromatograph and is a good indicator for any other labs out there that you got you call them clean. Unknown Speaker 23:38 I heard that you're working on something called antibiotic fusion technique. Unknown Speaker 23:45 Can you explain what this is and how this makes it more potent? So like I was saying before, when I first came into mycology, I was asking how to make fusion. And you know, everybody was like, either get out of here, it's not possible. Or I got directed to some places where it seemed there was some very kind of not well documented research on snake venom. Well, I looked all into it. You know, it said that it would work. I tried to paint some I couldn't get it. I don't even know where to get it still to this day, five years later, sick. No, actually, it's like seven, eight years later. And so one thing that everybody has, in their medicine cabinets is antibiotics. A lot of us use antibiotics in our ag guard to stave off contaminations. There's different kinds of antibiotics get a little technical, but some is called gram negative gram positive, they have different action against either bacteria or molds. So a lot of them get their biotics that we use in mycology, like gentle myosin or camp myosin. They only have a one gram negative action. So a lot of times it'll have very similar effect just like activated charcoal. When you put it on your ag bar. What it'll do is the you'll get to rise Unknown Speaker 25:00 dimorphic strains pop off and why to the sides. And that's because they're trying to stay away from that antibiotic resistance markers there and get away from them. But with the antibiotics that I use was straight into my medicine cabinet, I use the first I use the penicillin and I experimented with amoxicillin. Now I use chloramphenicol, because they have gram positive and negative action. And it was very cheap. And it was very easy to find now and it has both gram negative and positive action, the mycelium doesn't shoot all in the directions, like I said, it just stays in one spot and grows really, really slow. Because no matter where it goes, it's like it doesn't want to go there. So when you put too, to say mono carry out extremes where they can even be die carry out experience onto this or like an isolation, like sandwich together onto the same plate, what it does is it makes them kind of homogenized and melt in that spot because it doesn't want to it grows very slow, it doesn't want to grow fast. So it was very, very slow. And it kind of forces them to fuse and make clamp connections. Now that was the way that I started doing it in the beginning, since we've found some better ways like in these liquid suspensions, which is now in 3d, right. So you think like your Adguard plane is just flat, so it's two dimensional. So in a liquid suspension, it's 3d. So now it should grow. It could grow out in whatever direction you want. And in doing some of other companies experiments, like the Odin, and I'll throw his name out there, because he just rocks they got all these genetic kits, like added do like low and mushroom kits and CRISPR kits and all this stuff, I was using all his stuff and some other companies stuff. And they all came with this transformation buffer. And I was messing around at the time with trying to make a glowing strain. And we're still doing it now. And we use the same fusion buffer. So basically what this fusion Buffer does is the same thing that the antibiotic does, basically soften that cell wall and make it so that the the two high phase can clamp together and kind of meld and then what's being transferred between them is the nucleus which contains the genetic information. Now when we do DNA, or genetic kits, we're usually implanting something like like a DNA plasmid, which is in a circle. So we use this fusion buffer, which would say break down the bacteria a little bit that cell wall so that that so that that plasmid DNA can slip through the wall and get into the nucleus of the cell. And we well this time we've been doing it with hyphae like I mentioned, like mono carry ons, and I carry on you can use it where I got the idea to do it was in a very famous book by Paul Stamets mushroom cultivator, the blue book, it's like it's like one of our textbooks. And right it was one of the first books I read, and right there on page 27, he's demonstrating it with the Ikarians no less with four different Ikarians on the page. So I recommend everybody go check out page 27 of mushroom cultivation, and you'll see exactly where I got the idea right there. So that was with di Ikarians. Right, so and they can be tried Cheerios, and there's all kinds of genetic six systems is Tetra polar, and all these other Unknown Speaker 28:16 unit factorable and bifactor. All and you guys can go look up what all these things mean. But um, but but since we do them with monos, we do them with with the dye carry ons, but since now we're trying with sports, because we haven't been very successful with inserting that glowing GFP plasmid DNA into hyphae. Man I did, but it wasn't, it wasn't. It wasn't hereditary. In other words, it didn't get into the gene line, the germ line, it just died right there. It was a little glowy for a minute. And then once it makes new nucleus, it just they're not glowing. So my theory is that by trying it with the spores, you know, we're now it's pre germination. So we're getting it right it right in the beginning. And the cell wall should be very easy to get through. We'll we'll we'll get through it there and insert the plasmid DNA then and see if it has the see if we can get it to glow. So in a similar way, we do a fusion experiments with the same kind of fusion buffers, it's a proprietary blend, but it's basically built made with water and calcium fluoride. And it just kind of breaks down the cell wall but doesn't kill you know, the helping leaves, Celia Unknown Speaker 29:26 is Is this the same as Protoplast fusion? Or is it a bit different? Unknown Speaker 29:32 Maybe a bit too. Cool. And what do you what are you hoping to accomplish with it besides, you know, making cool glow in the dark, like, is there Unknown Speaker 29:45 can you put other you know, like functional compounds that one reason why I wanted to do any of this was to prove my work. So I was making these hybrids and you know, half half the community was saying, Oh, it looks like that one and the other half were saying no, it looks like the other one. Unknown Speaker 30:00 And then like so it was like, did it work or did it not? So all of this was in in, in hopes of us of just doing better documentation and and better work. Unknown Speaker 30:13 So Unknown Speaker 30:16 I'm sorry, boss for training Unknown Speaker 30:19 the team you do you think that Unknown Speaker 30:22 is there any hopes for CRISPR or any kind of genetic modification of fungi? Do you think that has a place in the psilocybin industry Unknown Speaker 30:38 possibly so that's what I was getting that the reason why I wanted to do that was to prove my work. So I figured if I can make one string glow red and one string cloak green, it would be very easy to show that to fusing together as we can look at it under some kind of light, kind of UV light and just show so that was the reason why I started it you know, what would I be looking to get out? You know, I'm not looking through. Unknown Speaker 31:03 Like you were saying make oysters make psilocybin you know, I don't know if that would if that would be a thing. I know that there is a tech out there for fermenting Unknown Speaker 31:15 psilocybin and big vats with with bacteria Unknown Speaker 31:20 serve sei the Yeah. And they were seized yet totally with you. So with yeast so basically, we would do the same thing like with the glowing stuff, but we would basically make it make psilocybin instead of Unknown Speaker 31:35 Yeah. So and that is that is a tag that is out there. I don't know if anybody's doing it there is a tech on line to do it in one liter vessels or two liter vessels. I haven't tried it myself believe very complicated, but I could see something like that happen in the future like where big government tries to like just make big vats of this stuff and then write for single compound use Yeah. Unknown Speaker 31:59 I'm not sure how exactly how I feel about that. But um, but for myself, like it was just for fun just to prove our work to make fusions a glowing string sound good fun. I made my own corn my own rainbow corn. That's how I learned how to do the CRISPR with the cast nine Unknown Speaker 32:18 what wouldn't you What would you say is the hardest part of this this journey so far? Unknown Speaker 32:25 On the hardest part Unknown Speaker 32:31 the hardest part is probably just, I I've grown a lot since I started right. So probably the hardest part is Unknown Speaker 32:42 just just keeping up with all the work man because I'm one guy and I do everything by myself. So like I really wish I had in the system sometimes. But I really don't think this is a hard hobby for like anybody to get into like this is I think it's even easier than cannabis to grow like you don't need a lot of space. You don't need a lot of light or fancy equipment. You just need like a grow bag and a syringe and you can get started you inoculate it, they liked the dark you can put it in your closet while it's colonizing and then when it's ready to fruit open up to the bag a little bit because coming rolled up and you know it's in a sealed environment. You never have to open it or worry about contamination it's a good way for a beginner to get a successful grow into their belt and get some confidence and then they could go try some more advanced techniques. One of the hardest things was really just for me to be successful in the beginning. So finding a good mentor. We're a good group Unknown Speaker 33:35 like trip teen family or magic myco fam or any other group out there positive influencers that you get involved with because there's some really good mentors out there. I learned tons from Willie myco going that first year and a half I was unsuccessful that I found Willie myco in my whole world turned around and within no time I was successful. So find a good girl find a good mentor, get some good books, mushroom cultivator radical mycology and you want having a hard time. This is probably one of the easiest medicines that you can grow for yourself. And it's probably one of the most important Unknown Speaker 34:10 what has been the most rewarding part of this journey for you. Unknown Speaker 34:15 The rewarding card the relationship I have with the mushrooms and the community. Like I said my fam I got a great team, I got a great family supporting me not only what mushrooms what were like, we talked about all kinds of stuff, you know, you name it. Unknown Speaker 34:30 So So my friends, my colleagues, the supporters Unknown Speaker 34:35 that burst they keep me going Unknown Speaker 34:38 and where can people follow your work? Yeah, you're talking about a Patreon in the beginning. Unknown Speaker 34:44 Yeah, give give a shout out and where people can dive deeper and so magic myco.net We'll send it to the link tree with all the links you could go to magic myco.com for the bending site, you could go to magic myco.org. For all the testing information. You can Unknown Speaker 35:00 Email me DOMA at magic myco.org. Anytime if this cult of our cup related you can email cult of our cup@gmail.com. Unknown Speaker 35:10 Great, awesome. Well any any. Unknown Speaker 35:16 To wrap it up what would you say is Unknown Speaker 35:20 in the next 510 years what would you say is your hopes for this industry, Unknown Speaker 35:26 legalization, legalization and Colette is like mine to be everywhere but cultivators to be everywhere for it to grow on base in and out of time. While you guys to get out there and making your own medicine five years from now. It should be legalized everybody. There's good bills out there that you can get involved with corto Tech is one of my good friends and he has a free Patreon, you should check out his Patreon and a post he made recently about legalization and all the bills going on in the United States. You could click on the link, see all the bills that are active in your areas, get in touch with the legislative people in your area and see what you could do to get involved on the bill in your area and get this stuff legalized. Unknown Speaker 36:08 Awesome. Cool. Well, thank you. And Unknown Speaker 36:12 thanks for everyone for tuning in and trimming into another episode. Check out all the links that David just just talked about. We'll have we'll have a bunch in the bio. And yeah, wherever you're tuning in from from around the world. If you want to support the show, please leave a review and tell your friends tell your family tell a stranger on the street. And if you want to financially support the show, we don't have a Patreon or anything like that. But we do have a site mushroom revival.com where we have you know, tinctures capsules, gummies powders of a bunch of functional mushroom species. So if you want to get it for yourself or a friend family member, check it out there. We also have a bunch of free ebooks and blogs and recipes and a bunch of fun stuff on there as well. So check it out. And as always much love and maybe spores be with you Transcribed by https://otter.ai